+91-11-45201100 drjindal@rdgc.in
SARS-CoV-2 Nucleic Acid Detection
Assay Principle:
The primer-probe mix adopts the design, targeting specific and nucleoprotein gene. With the amplification of template can be monitored by the increasing fluorescence signal detected by a real time PCR instrument. The PCR detection system includes internal control and primer probe mix . The result of internal control provides the accuracy of sampling and extraction process, in order to avoid false negative results.
Primers and probes are designed to detect conserved sequence encoding the ORF lab gene and N gene in the viral RNA simultaneously
Minimum detection limit is 500 copies/ml
Enzyme mix is lyophilized to achieve a longer shelf-life after opening, compared to liquid reagent
Single round of PCR completed within 1 hr to improve detection efficiency.
Compatible with applied biosystems 7500/ 7500FAST Roche LightCycler 480, and Shanghai Hongshi SLAN-96P, and Real-Time PCR instruments with FAM, HEX/VIC, and RED/ROX channels